MABN604
Anti-LAR/PTPRF Antibody, clone N165/38
clone N165/38, from mouse
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Receptor-type tyrosine-protein phosphatase F, Leukocyte common antigen related, LAR
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N165/38, monoclonal
species reactivity
rat
technique(s)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PTPRF(5792)
General description
Receptor-linked protein-tyrosine phosphatases (RPTPs) are signaling proteins that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. Leukocyte common antigen receptor protein tyrosine phosphatase (LAR/PTPRF), a negative regulator of several receptor tyrosine kinases, is present in high concentrations in mature synapses and is considered to be important for the development and maintenance of excitatory synapses in hippocampal neurons. Disruption of LAR function is shown to result in loss of excitatory synapses, diminution in surface AMPA receptors, and impairment of dendritic targeting of the cadherin–beta-catenin complex. LAR is also present in neurons expressing TrkB where it participates in survival of neurons and the neurite outgrowth. Increased expression of LAR in insulin-responsive tissues has also been associated with obesity and insulin resistance.
Specificity
Other Homologies: Mouse (97% sequence homology).
This antibody does not cross-react with PTPRD and PTPRS (Prof. J. Trimmer, University of California, Davis.).
Immunogen
Recombinant protein corresponding to human LAR/PTPRF.
Application
Anti-LAR/PTPRF Antibody, clone N165/38 is a highly specific mouse monoclonal antibody & that targets LAR & has been tested in western blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Quality
Evaluated by Western Blotting in rat brain membrane tissue.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected LAR/PTPRF in 10 µg of rat brain membrane tissue.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected LAR/PTPRF in 10 µg of rat brain membrane tissue.
Target description
~78 kDa observed. The full length protein is 210 kDa; however, it runs lower in Western Blotting due to proteolysis into P-subunit containing transmembrane and intracellular domains (Prof. J. Trimmer, University of California, Davis).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat brain membrane tissue
Rat brain membrane tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Oncogene, 39(44), 6789-6801 (2020-09-26)
Wnt signaling dysregulation promotes tumorigenesis in colorectal cancer (CRC). We investigated the role of PTPRF, a receptor-type tyrosine phosphatase, in regulating Wnt signaling in CRC. Knockdown of PTPRF decreased cell proliferation in patient-derived primary colon cancer cells and established CRC
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