MABN59
Anti-ACE2 Antibody, clone 4G5.1
clone 4G5.1, from mouse
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Synonym(s):
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
tag
GST tagged
antibody form
purified antibody (protein G)
antibody product type
primary antibodies
clone
4G5.1, monoclonal
mol wt
~62 kDa
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ACE2(59272)
General description
Angiotensin-converting enzyme 2 (ACE2) is a transmembrane protein belonging to the metallopeptidases (M2) family. It is a membrane-associated carboxypeptidase, expressed in the vascular endothelial cells of the heart, kidney, testis, gastrointestinal system and in the epithelial cells of lungs. ACE2 consists of an N-terminal peptidase domain (PD) and a C-terminal collectrin-like domain (CLD). It also comprises a single catalytic domain that is similar to that of ACE. The gene ACE2 is mapped to human chromosome location Xp22.2. ACE2 is a structural homolog ACE.
Specificity
This antibody recognizes ACE2.
Immunogen
GST-tagged recombinant protein corresponding to human ACE2.
Application
Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.
ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Biochem/physiol Actions
Angiotensin-converting enzyme-2 (ACE2) produces angiotensin (1-9) by hydrolysis of angiotensin 1. Angiotensin (1-9) is a precursor for angiotensin (1-7). ACE2 may also directly produce angiotensin (1-7) by conversion of angiotensin II, which is involved in vasoconstriction.(2) Angiotensin (1-7) plays an important role in the renin-angiotensin system (RAS) signaling pathway and is involved in bradykinin-induced vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as lisinopril and captopril. This gene is upregulated in heart failure due to the overactivity of angiotensin II. ACE2 is studied for its potential therapeutic value for hypertension and heart failure. It binds to the viral envelope protein spike (S) protein and acts as a major entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 in human cells. Hence, it is considered as a potential therapeutic target for COVID-19. ACE2 exhibits protection from lung injury.
Quality
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.
Target description
~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.
Linkage
Replaces: MAB5676
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yuebo Zhang et al.
Frontiers in physiology, 9, 1370-1370 (2018-10-27)
Body fat distribution contributes to obesity-related metabolic and cardiovascular disorders. Visceral fat is more detrimental than subcutaneous fat. However, the mechanisms underlying visceral fat-mediated cardiometabolic dysregulation are not completely understood. Localized increases in expression of the renin angiotensin system (RAS)
Christoph Schindler et al.
Vascular health and risk management, 3(1), 125-137 (2007-06-23)
The renin-angiotensin-system (RAS) is a cascade of enzymatic reactions resulting ultimately in the formation of angiotensin II. Recent research has expanded the knowledge about the RAS by adding new components to the pathways: angiotensin-(1-5) [Ang-1-5], angiotensin-(1-7) [Ang-(1-7)], angiotensin-(1-9) [Ang-(1-9)], an
Denis S Ziablitsev et al.
Medicina (Kaunas, Lithuania), 59(4) (2023-04-28)
Background and Objectives: The coronavirus (SARS-CoV-2) damages all systems and organs. Yet, to a greater extent, the lungs are particularly involved, due to the formation of diffuse exudative inflammation in the form of acute respiratory distress syndrome (ARDS) with next
Mir S Adil et al.
Drug discoveries & therapeutics, 14(5), 256-258 (2020-10-30)
In the ongoing coronavirus diseases-2019 (COVID-19) crisis that caused immense suffering and deaths, the choice of therapy for the prevention and life-saving conditions must be based on sound scientific evidence. Uncertainty and apprehension are exacerbated in people using angiotensin-converting enzyme
Prasun K Datta et al.
Theranostics, 10(16), 7448-7464 (2020-07-10)
The COVID-19 pandemic is an emerging threat to global public health. While our current understanding of COVID-19 pathogenesis is limited, a better understanding will help us develop efficacious treatment and prevention strategies for COVID-19. One potential therapeutic target is angiotensin
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