MABN505
Anti-Pink1 Antibody, clone N4/15
clone N4/15, from mouse
Synonym(s):
Serine/threonine-protein kinase PINK1, mitochondrial, BRPK, PTEN-induced putative kinase protein 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N4/15, monoclonal
species reactivity
human, rat
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PINK1(65018)
General description
Mutations in the PTEN-induced kinase 1 (PINK1) gene located within the PARK6 locus on chromosome 1p35-p36 have recently been identified in patients with recessive early-onset Parkinson′s disease. Data suggests that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance.
Specificity
This antibody recognizes the cytoplasmic domain of Pink1. Other Homologies: Mouse (81% Sequence Homology) and Rat (82% Sequence Homology)
Immunogen
Epitope: Cytoplasmic domain
Recombinant protein corresponding to the cytoplasmic domain of human Pink1.
Application
Anti-Pink1 Antibody, clone N4/15 is a highly specific mouse monoclonal antibody & that targets PINK1 & has been tested in western blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pink1 in 10 µg of human testis tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Pink1 in rat caudate putamen tissue (Prof. J. Trimmer, University of California, Davis.).
Immunohistochemistry Analysis: A representative lot detected Pink1 in rat caudate putamen tissue (Prof. J. Trimmer, University of California, Davis.).
Quality
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pink1 in 10 µg of human skeletal muscle tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pink1 in 10 µg of human skeletal muscle tissue lysate.
Target description
~60 kDa observed. Uniprot describes two isoforms at 63 kDa and 30 kDa The full length protein of Pink1 is observed at 63 kDa is the full length PINK1; however, it is proteolytically cleaved to a ~52 kDa form upon entry into the mitochondria (Jin, S. M., et al. (2010). J Cell Biol. 191(5):933-942.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human skeletal muscle tissue lysate
Human skeletal muscle tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Arash Aghajani Nargesi et al.
American journal of hypertension, 33(8), 765-774 (2020-03-18)
Mitochondria modulate endothelial cell (EC) function, but may be damaged during renal disease. We hypothesized that the ischemic and metabolic constituents of swine renovascular disease (RVD) induce mitochondrial damage and impair the function of renal artery ECs. Pigs were studied
Yi Zhang et al.
Journal of immunology (Baltimore, Md. : 1950), 192(11), 5296-5304 (2014-04-30)
High levels of inspired oxygen, hyperoxia, are frequently used in patients with acute respiratory failure. Hyperoxia can exacerbate acute respiratory failure, which has high mortality and no specific therapies. We identified novel roles for PTEN-induced putative kinase 1 (PINK1), a
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