MABN476
Anti-Paris/ZNF746 Antibody, clone N196/16
clone N196/16, from mouse
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Synonym(s):
Zinc finger protein 746, Parkin-interacting substrate, PARIS
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N196/16, monoclonal
species reactivity
mouse, human, rat
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ZNF746(155061)
General description
Zinc finger protein 746 (ZNF746) is also called Parkin-interacting substrate (PARIS). ZNF746 is a repressor of transcription that binds to promoters and represses the transcription of PGC-1-alpha (PPARGC1A), making it important to the regulation of neuron death. ZNF746 may also contribute to neurodegeneration in Parkinson disease.
Specificity
This antibody recognizes the N-terminus of ZNF746. This antibody does not cross react with ZNF783 or ZNF398 (Prof. J. Trimmer, University of California, Davis.).
Immunogen
Epitope: N-terminus
Recombinant protein corresponding to the N-terminus of human Paris/ZNF746.
Application
Anti-Paris/ZNF746 Antibody, clone N196/16 is a highly specific mouse monoclonal antibody & that targets Zinc Finger Protein & has been tested in western blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of RAW264.7 cell lysate. Immunohistochemistry Analysis: A representative lot detected Paris/ZNF746 in rat brain tissue (Prof. J. Trimmer, University of California, Davis.).
Quality
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Paris/ZNF746 in 10 µg of HEK293 cell lysate.
Target description
~72 kDa observed. Uniprot describes 3 isoforms, two at 69 kDa and one at 49 kDa This protein is ubiquitinated and has been observed at higher molecular weights (Shin, J. H., et al. (2011). Cell. 144(5):689-702.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Benjamin P Woodall et al.
JCI insight, 5 (2019-04-17)
The E3 ubiquitin ligase Parkin plays an important role in regulating clearance of dysfunctional or unwanted mitochondria in tissues, including the heart. However, whether Parkin also functions to prevent cardiac aging by maintaining a healthy population of mitochondria is still
Milena Pinto et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(4), 1042-1053 (2017-12-10)
PARK2 is the most common gene mutated in monogenic recessive familial cases of Parkinson's disease (PD). Pathogenic mutations cause a loss of function of the encoded protein Parkin. ParkinKO mice, however, poorly represent human PD symptoms as they only exhibit
Hojin Kang et al.
Oncotarget, 8(30), 48603-48618 (2017-06-15)
Progressive dopaminergic neurodegeneration is responsible for the canonical motor deficits in Parkinson's disease (PD). The widely prescribed anti-diabetic medicine metformin is effective in preventing neurodegeneration in animal models; however, despite the significant potential of metformin for treating PD, the therapeutic
Ju-Hyeon Bae et al.
Cell death & disease, 11(5), 359-359 (2020-05-14)
Various stresses, including oxidative stress, impair the proliferative capacity of muscle stem cells leading to declined muscle regeneration related to aging or muscle diseases. ZNF746 (PARIS) is originally identified as a substrate of E3 ligase Parkin and its accumulation is
Yi Zhang et al.
Journal of immunology (Baltimore, Md. : 1950), 192(11), 5296-5304 (2014-04-30)
High levels of inspired oxygen, hyperoxia, are frequently used in patients with acute respiratory failure. Hyperoxia can exacerbate acute respiratory failure, which has high mortality and no specific therapies. We identified novel roles for PTEN-induced putative kinase 1 (PINK1), a
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