MABN464
Anti-Cav3.1 Ca2+ channel Antibody, clone N178A/9
clone N178A/9, from mouse
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All Photos(3)
Synonym(s):
Voltage-gated calcium channel, alpha-1-G subunit
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N178A/9, monoclonal
species reactivity
mouse, rat
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Cacna1G(12291)
General description
Voltage-dependent T-type calcium channel subunit alpha-1G or Voltage-gated calcium channel subunit alpha Cav3.1, like other members of the voltage-sensitive calcium channel (VSCC) family, mediate the entry of calcium ions into excitable cells. These channels regulate and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and also support calcium signaling in secretory cells and vascular smooth muscle.
Specificity
Other homologies: Human (80% sequence homology).
Immunogen
Recombinant protein corresponding to mouse Cav3.1.
Application
Immunohistochemistry Analysis: 1 µg/mL from a representative lot detected Cav3.1 Ca2+ channel in rat thalamus tissue.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in mouse thalamus, cortex, and cerebellum tissue.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in rat hippocampus tissue.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate and in WT mouse brain membrane tissue lysate, but demonstrates a loss of signal in Cav3.1 Ca2+1 knockout mouse brain membrane tissue lysate.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in mouse thalamus, cortex, and cerebellum tissue.
Immunohistochemistry Analysis: A representative lot detected Cav3.1 Ca2+ in rat hippocampus tissue.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate.
Western Blot Analysis: A representative lot detected Cav3.1 Ca2+ in rat brain membrane tissue lysate and in WT mouse brain membrane tissue lysate, but demonstrates a loss of signal in Cav3.1 Ca2+1 knockout mouse brain membrane tissue lysate.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Cav3.1 Ca2+ channel antibody is validated for use in WB & IHC for the detection of the Cav3.1 Ca2+ channel protein.
Quality
Evaluated by Western Blot in mouse cerebellum tissue.
Western Blot Analysis: 1 µg/mL of this antibody from a representative lot detected Cav3.1 Ca2+ channel in 10 µg/mL of mouse cerebellum tissue.
Western Blot Analysis: 1 µg/mL of this antibody from a representative lot detected Cav3.1 Ca2+ channel in 10 µg/mL of mouse cerebellum tissue.
Target description
~230 kDa observed. An uncharacterized band at ~53 kDa may be observed in some tissue lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse cerebellum tissue
Mouse cerebellum tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Paula P Perissinotti et al.
Frontiers in neuroscience, 15, 679078-679078 (2021-06-29)
Leptin regulates hypothalamic POMC+ (pro-opiomelanocortin) neurons by inducing TRPC (Transient Receptor Potential Cation) channel-mediate membrane depolarization. The role of TRPC channels in POMC neuron excitability is clearly established; however, it remains unknown whether their activity alone is sufficient to trigger
Marcin Andrzej Lipiec et al.
Development (Cambridge, England), 147(16) (2020-07-18)
Neuronal phenotypes are controlled by terminal selector transcription factors in invertebrates, but only a few examples of such regulators have been provided in vertebrates. We hypothesised that TCF7L2 regulates different stages of postmitotic differentiation in the thalamus, and functions as
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