Anti-nonphospho-Ser9/21 GSK3β/α Antibody, clone 15C2

Glycogen synthase kinase-3 beta/alpha (UniProts: P49841 and P49840; also known as EC: 2.7.11.26 and 2.7.11.26, GSK-3 beta/alpha, Serine/threonine-protein kinase GSK3B/alpha) is encoded by the GSK3B/GSK3A gene (Gene ID: 2932/2931) in human. GSK-3 is a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways during embryogenesis and in metabolic control. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3 alpha and a 47 kDa GSK-3 beta. The GSK-3 alpha contains a glycine-rich extension at its N-terminal. GSK-3 is constitutively active in cells and is regulated through inhibition of its activity. GSK-3 shows a preference for target proteins that are pre-phosphorylated at a `priming residue located C-terminal to the site of GSK-3 phosphorylation. Priming phosphorylation, although not absolutely required, enhances the efficiency of phosphorylation of most GSK-3 substrates. Phosphorylation of a threonine residue in the activation loop (T-loop) is considered to be essential for its activity. The T-loop of GSK-3 alpha is phosphorylated at Tyr279 and GSK-3 beta at Tyr216, which play a role in forcing open the substrate-binding site of the enzyme. A primary mechanism of GSK3 activity regulation is phosphorylation of N-terminal serine residues (Serine 9 in GSK3 beta and serine 21 in GSK3 alpha). Phosphorylation of these sites is inhibitory to GSK3 kinase activity because the phosphorylated N-terminus acts as a competitive inhibitor for primed substrates. GSK-3 beta is constitutively active in resting cells and treatment of cells with an agent, such as insulin, causes GSK-3 inactivation through a PI 3-kinase (PI 3-K)-dependent mechanism.

Clone 15C2 detects non-phosphorylated forms of both GSK-3 beta and GSK-3 alpha in Human, Mouse, and Rat. It targets an epitope within the N-terminal region.

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human GSK-3 beta.

Anti-nonphospho-Ser9/21 GSK3 beta/alpha, clone 15C2, Cat. No. MABN2465, is a highly specific mouse monoclonal antibody that targets nonphospho Ser9/21 GSK3 beta/alpha and has been testedfor use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected nonphospho-Ser9/21 GSK3 / in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected nonphospho-Ser9/21 GSK3 / in human cerebral cortex and rat cerebral cortex tissues.

Immunoprecipitation Analysis: A representative lot detected nonphospho-Ser9/21 GSK3 / in Immunoprecipitation applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Western Blotting Analysis: A representative lot detected nonphospho-Ser9/21 GSK3 / in Western Blotting applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunocytochemistry Analysis: A representative lot detected nonphospho-Ser9/21 GSK3 / in Immunocytochemistry applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Immunohistochemistry Analysis: A representative lot detected nonphospho-Ser9/21 GSK3 / in Immunohistochemistry applications (Grabinski, T., et. al. (2016). Front Mol Neurosci. 9:123).

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected nonphospho-Ser9/21 GSK3 beta/alpha in 10 µg of rat brain tissue lysate.

46.74/50.98 kDa calculated for GSK3 beta and alpha, respectively. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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