Anti-phospho-NHE3 (Ser605) Antibody, clone 10A8

Sodium/hydrogen exchanger 3 (UniProt: P26433; also known as Na(+)/H(+) exchanger 3, NHE-3, Solute carrier family 9 member 3) is encoded by the Slc9a3 (Nhe3) gene (Gene ID: 24784) in rat. NHE-3 is a multi-pass membrane protein that is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It is a major proton extruding system driven by the inward sodium ion chemical gradient. NHE-3 is shown to be most abundant in colon and small intestine and is also present in stomach and in proximal tubes and outer medulla of kidney. In kidneys it mediates the majority of sodium bicarbonate reabsorption by the proximal tubules. NHE-3 can be phosphorylated and regulated by multiple kinases, including PKA, SGK1, and CK2 and its phosphorylation is critical in its regulation. Parathyroid hormone is shown to enhance PKA-mediated phosphorylation of Serine 605, however, this phosphorylation does not affect the activity of NHE-3. (Ref.: Kocinsky, HS., et al. (2007). Am J Physiol Renal Physiol 293 (1); F212-F218).

This mouse monoclonal antibody (clone 10A8) specifically detects Sodium/hydrogen exchanger 3 phosphorylated on Serine 605.

KLH-conjugated linear peptide corresponding to 22 amino acids surrounding phospho Serine 605 from the C-terminal half of rat Sodium/ hydrogen exchanger 3 (NHE3).

Anti-phospho-NHE3 (Ser605), clone 10A8, Cat. No. MABN2414, is a highly specific mouse monoclonal antibody that targets Sodium/hydrogen exchanger 3 and has been tested for use in ELISA and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected phospho-NHE3 (Ser605) in Western Blotting applications (Dynia, D.W., et. al. (2010). Am J Physiol Reanl Physiol. 298(3):F745-53; Kocinsky, H.S., et. al. (2005). Am J Physiol Reanl Physiol. 289(2):F249-58; Kocinsky, H.S., et. al. (2007). Am J Physiol Reanl Physiol. 293(1):F212-8).

ELISA Analysis: A representative lot detected phospho-NHE3 (Ser605) in ELISA applications (Kocinsky, H.S., et. al. (2005). Am J Physiol Reanl Physiol. 289(2):F249-58).

Evaluated by Western Blotting in COS-7 cells either transfected with wild type NHE3, mutated NHE3 (S605G), or untransfected COS-7 (WT) cell lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Sodium/hydrogen exchanger 3 in lysate from COS-7 cells transfected with wild- type NHE-3 (pSer605). It did not detect NHE-3 in lysate from untransfected COS-7 cells or those transfected with mutated NHE-3 (S605G).

~85 kDa observed; 93.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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