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MABN2185

Sigma-Aldrich

Anti-LGMN Antibody, clone 6A7.2

clone 6A7.2, from mouse

Synonym(s):

Asparaginyl endopeptidase, Protease cysteine 1, Legumain

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

6A7.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001008530

UniProt accession no.

Q99538

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... LGMN(5641)

General description

Legumain (UniProt: Q99538; EC:3.4.22.34, also known as Asparaginyl endopeptidase; Protease, cysteine 1) is encoded by LGMN (also known as PRSC1) gene (Gene ID 5641) in human. Legumain is a lysosomal enzyme with strict specificity of the hydrolysis of asparaginyl bonds. It is shown to be ubiquitously distributed with abundance in kidney, heart, and placenta. In the zymogen form, the uncleaved propeptide blocks access to the active site. It is activated autocatlytically by acidic pH and the active form is shown to be monomeric. Legumain plays a pivotal role in the endosomal/lysosomal degradation system and is involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation. Legumain deficiency causes the accumulation of pro-cathepsins B, H and L. Overexpression of legumain is associated with invasion and metastasis in a majority of solid tumors.

Specificity

Clone 6A7.2 of detects LGMN in multiple human tissues by Western blotting and immunohistochemistry.

Immunogen

GST-tagged recombinant fragment corresponding to 95 amino acids from the internal region of human LGMN.

Application

Anti-LGMN, clone 6A7.2 Antibody, Cat. No. MABN2185, is a highly specific mouse monoclonal antibody that targets LGMN and has been tested in Western Blotting and Immunohistochemistry (Paraffin).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LGMN in human placenta, human kidney, and human small intestine.


Quality

Evaluated by Western Blotting in human heart tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected LGMN in 10 µg of human heart tissue lysate.

Target description

~ 55 kDa observed; 49.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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