Anti-HCN1 Antibody, clone N70/28

HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 proteins play an important role for motor learning and synapse formation through integration of cerebellar Purkinje cells. The two proteins also shape autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is particularly high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization of activated cation channels of the HCN gene family such as HCN1, contributes to spontaneous rhythmic activity in both heart and brain. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore.

This antibody recognizes HCN1 at the cytoplasmic domain.

Epitope: Cytoplasmic domain

Recombinant protein corresponding to rat HCN1 at the cytoplasmic domain.

Detect HCN1 using this Anti-HCN1 Antibody, clone N70/28 validated for use in WB, IH.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected HCN1 in rat cerebellum and rat brain/inferior colliculus tissue.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Evaluated by Western Blot in rat brain lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected HCN1 on 10 µg of rat brain lysate.

~120 kDa observed; consistent with a glycosylation of a 100kDa protein.

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat brain lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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