Anti-Neuregulin-2 Antibody (NRG2), clone 8D11

Pro-neuregulin-2, membrane-bound isoform (UniProt P56974; also known as Pro-NRG2) is encoded by the Nrg2 gene (Gene ID 100042150) in murine species. The ErbB4 receptor is widely expressed in GABAergic interneurons and reply on the engagement of neuregulins (NRGs) as ligands for signaling during neural processes in the adolescent and adult central nervous system (CNS). NRG1 transcript is generally lower than NRG2 in postnatal CNS and NRG2 is a major functional ErbB4 ligand in the postnatal brain. NRGs are synthesized as single-pass transmembrane pro-forms that is posttranslationally converted to signalling ligands by extracellular sheddases. The presence of an extracellular immunoglobulin (Ig)-like domain allows NRG1/2 ectodomain to accumulate near the site of cleavage through interactions with extracellular matrix heparan proteoglycans. NMDARs are required to release the NRG2 ectodomain from the surface of interneuron cell bodies. Stimulation of ErbB4 by the NRG2 ectodomain not only promotes the internalization of ion channels from the cell surface, but also the association of ErbB4 with GluN2B-containing NMDARs, resulting in a rapid downregulation of NMDARs on inhibitory interneurons, but not pyramidal neurons.

Clone 8D11 epitope lies within neuregulin-2, which corresponds to the extracellular domain of membrane-bound pro-neuregulin-2.

Recombinant mouse mature Neuregulin-2.

Anti-Neuregulin-2 (NRG2), clone 8D11, Cat. No. MABN1853, is a highly specific mouse monoclonal antibody that targets Neuregulin-2 (NRG2) and has been tested in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin).

Immunocytochemistry Analysis: A representative lot detected somatodendritic pro-NRG2 puncta on the surface of cultured rat hippocampal ErbB4+ interneurons (DIV 21 & 28) fixed with 4% paraformaldehyde without permeabilization (Vullhorst, D., et al. (2015). Nat. Commun. 6:7222).

Immunofluorescence Analysis: A representative lot detected NRG2 immunoreactivity among ErbB4+ interneurons in CA1 strata pyramidale and radiatum of 4% paraformaldehyde-fixed, 0.25% Triton X-100-permeabilized rat brain sections (Vullhorst, D., et al. (2015). Nat. Commun. 6:7222).

Research Category
Neuroscience

Evaluated by Immunohistochemistry in human cerebellum tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Purkinje cells surface Neuregulin-2 (NRG2) immunoreactivity in human cerebellum tissue sections.

80.30/67.13/70.64 (human pro-neuregulin-2 isoform 1/DON-1B/DON-1R), 32.60/19.43/22.94 (human neuregulin-2 isoform 1/DON-1B/DON-1R), 80.17/80.04 kDa (mouse pro-neuregulin-2 isoform NRG2-16A/DON-1M), 32.66/32.53/34.24/28.66 kDa (mouse neuregulin-2 isoform NRG2-16A/DON-1M/DON-1S (NRG2-5)/NRG2-10), 80.79/79.84/79.97/79.01/28.63/34.34/79.96 kDa (rat pro-neuregulin-2 isoform NTAK-alpha1/NTAK-alpha2A/NTAK-alpha2B (NTAK-alpha2-1P)/NTAK-beta/NTAK-gamma/NRG2-alpha/NRG2-beta), 33.46/32.50/32.63/31.67/28.63/34.34/32,63 kDa (rat neuregulin-2 isoform NTAK-alpha1/NTAK-alpha2A/NTAK-alpha2B (NTAK-alpha2-1P)/NTAK-beta/NTAK-gamma/NRG2-alpha/NRG2-beta) calculated.

Format: Purified

Protein G purified.

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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