MABN1795
Anti-Progranulin/GRN Antibody, clone 8H10
clone 8H10, from rat
Sign Into View Organizational & Contract Pricing
All Photos(1)
Granulins, Progranulin/GRN
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8H10, monoclonal
species reactivity
mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2cκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Grn (14824)
General description
Granulins constitute a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 602 aa murine precursor protein, preprogranulin (UniProt Q3U9N4), is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide (aa 1-17) produces progranulin (Acrogranin; aa 18-589), which can be further cleaved into 7 active peptides named granulin-1 through granulin-7. Both the peptides and progranulin protein regulate cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Heterozygous loss-of-function mutations in the GRN gene is a known cause for frontotemporal lobar degeneration (FTLD) with accumulation of TAR DNA-binding protein (TDP)-43, while a complete GRN deficiency due to a homozygous loss-of-function mutation causes the lysosomal storage disorder known as neuronal ceroid lipofuscinosis (NCL). In Grn-/- mice, a murine model for GRN-associated FTLD-TDP (FTLD-TDP/GRN), the lysosomal proteins cathepsin D (CTSD) is found to be elevated, while an upregulated granulin level is observed in Ctsd(−/−) mice, a model for NCL. Moreover, both types of knockout mice are found to display increased levels of transmembrane protein 106B (TMEM106B) as a common phenotype.
Specificity
Reacts with Preprogranulin and Progranulin (Acrogranin, Proepithelin), but not Granulin-1 through Granulin-7.
Immunogen
Epitope: Near C-terminus
Linear peptide corresponding to rat progranulin sequence near the C-terminus
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Progranulin/GRN Antibody, clone 8H10 is validated for use in Western Blotting, Immunohistochemistry for the detection of Progranulin/GRN.
Western Blotting Analysis: A representative lot detected elevated progranulin expression in brain homogenates from Ctsd-/- mice, and loss of progranulin expression in brain homogenates from Grn-/- mice (Gotzl, J.K., et al (2014). Acta Neuropathol.127(6):845-860).
Immunohistochemistry Analysis: A representative lot detected elevated progranulin expression in the cortex, hippocampus, and thalamus regions of Ctsd-/- mice when compared with wild-type mice using frozen brain sections (Gotzl, J.K., et al (2014). Acta Neuropathol.127(6):845-860).
Immunohistochemistry Analysis: A representative lot detected elevated progranulin expression in the cortex, hippocampus, and thalamus regions of Ctsd-/- mice when compared with wild-type mice using frozen brain sections (Gotzl, J.K., et al (2014). Acta Neuropathol.127(6):845-860).
Quality
Evaluated by Western Blotting in mouse uterus tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Progranulin/GRN in 10 µg of mouse uterus tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected Progranulin/GRN in 10 µg of mouse uterus tissue lysate.
Target description
~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2cκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service