MABN1616
Anti-GADD153/DDIT3/CHOP Antibody, clone 5E5.3
clone 5E5.3, from mouse
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DNA damage-inducible transcript 3 protein, CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5E5.3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... CEBPZ(10153)
General description
DNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (Also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.
Specificity
Clone 5E5.3 targeted region is present in both human GADD153/DDIT3/CHOP spliced isoforms reported by UniProt (P35638).
Immunogen
Epitope: Internal.
GST-tagged recombinant human GADD153/DDIT3/CHOP internal fragment.
Application
Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected GADD153/DDIT3 in HeLa cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GADD153/DDIT3 in human brain, kidney, and skeletal muscle tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GADD153/DDIT3 in human brain, kidney, and skeletal muscle tissue sections.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-GADD153 Antibody, clone 5E5.3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of CHOP.
Quality
Evaluated by Western Blotting Analysis in HUVEC lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected GADD153/DDIT3 in 10 µg of HUVEC lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected GADD153/DDIT3 in 10 µg of HUVEC lysate.
Target description
~20 kDa observed. 19.18 kDa (isoform 1) and 21.70 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Physical form
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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