MABN1537
Anti-TDO2 Antibody, clone 7A6.1
clone 7A6.1, from mouse
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Synonym(s):
Tryptophan 2, 3-dioxygenase, TDO, TO, TRPO, Tryptamin 2, 3-dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7A6.1, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TDO2(6999)
General description
Tryptophan 2,3-dioxygenase (EC 1.13.11.11; UniProt P48775; also known as TDO, TO, TRPO, Tryptamin 2,3-dioxygenase, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase) is encoded by the TDO2 (also known as TDO) gene (Gene ID 6999) in human. TDO2 is a cytosolic heme-containing enzyme that exists as a homotetramer, where the N terminal residues of each monomer form part of the substrate binding site in an adjacent monomer. Tryptophan 2,3-dioxygenase (TDO2) catalyzes the first step of the kynurenine pathway, namely the conversion of tryptophan to kynurenine (Kyn), which can also be catalyzed by indoleamine 2,3-dioxygenase 1 (IDO1) or IDO2. IDO1 is expressed in tissues throughout the body, while TDO2 is predominantly expressed in the liver. Altered tryptophan metabolism and increased secretion of tryptophan metabolites are found in solid tumors, including in breast cancer. TDO2-mediated Kyn production in triple-negative breast cancer (TNBC) cells is reported to activate aryl hydrocarbon receptor (AhR), an endogenous kynurenine receptor. Inhibiting TDO2 or AhR via pharmacologic or genetic means is reported to result in increased sensitivity to anoikis, as well as decreased proliferation, migration, and invasion of TNBC cells.
Specificity
Clone 7A6.1 targets an internal epitope of human and murine TDO2 (TDO).
Immunogen
GST-tagged recombinant human TDO2 internal fragment.
Application
Anti-TDO2, clone 7A6.1, Cat. No. MABN1537, is a highly specific mouse monoclonal antibody that targets TDO2, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TDO2 in human kidney, liver, and small intestine tissue sections.
Quality
Evaluated by Western Blotting in mouse liver tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TDO2 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected TDO2 in 10 µg of mouse liver tissue lysate.
Target description
~48 kDa observed. 47.87 kDa (human) and 47.76 kDa (mouse) calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Saeko Yoshioka et al.
International journal of tryptophan research : IJTR, 15, 11786469221138456-11786469221138456 (2022-12-06)
Kynurenine (Kyn), a metabolite of tryptophan (Trp), is a key regulator of mammal immune responses such as cancer immune tolerance. Indoleamine-2,3-dioxygenase (IDO) and tryptophan-2,3-dioxygenase (TDO) are main enzymes regulating the first and rate-limiting step of the Kyn pathway. To identify
Tatsuya Ando et al.
Molecular medicine reports, 27(2) (2022-12-10)
The partial loss of liver due to liver transplantation or acute liver failure induces rapid liver regeneration. Recently, we reported that the selective inhibition of indoleamine 2,3‑dioxygenase (Ido) 1 promotes early liver regeneration. However, the role of Ido2 in liver
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