MABN15
Anti-Rhodopsin Antibody, clone 4D2
clone 4D2, from mouse
Synonym(s):
rhodopsin, rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant), retinitis pigmentosa 4, autosomal dominant, opsin 2, rod pigment
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4D2, monoclonal
species reactivity
rat, human, fish
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RHO(6010)
rat ... Rho(24717)
Related Categories
General description
Rhodopsin is a multi-pass, G-protein-coupled membrane receptor protein belonging to the G-protein coupled receptor 1 family. Found within rod photoreceptor cells, rhodopsin is activated by light photons in order for the visual transduction cascade to initialize. Rhodopsin is necessary to the forming of images in low light and for the viability of photoreceptor cells after birth. Mutations in rhodopsin expression are the most widely known cause of the human disease retinitis pigmentosa (RP) resulting in gradual loss of vision. At present, there are over 100 known rhodopsin mutations.
Specificity
This antibody recognizes Rhodopsin at the N-terminus.
Immunogen
Epitope: N-terminus
Rat rod outer segments corresponding to rat Rhodopsin at the N-terminus.
Application
Anti-Rhodopsin Antibody, clone 4D2 is an antibody against Rhodopsin for use in IH, WB, IC, ELISA.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western Blot Analysis: A previous lot of this antibody detected Rhodopsin by WB in goldfish retina lysate, as reported by Knight K, et al.
Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al
Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.
ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.
Immunocytochemistry: A previous lot of this antibody detected Rhodopsin by ICC in goldfish retina tissue, as reported by Knight K, et al
Immunohistochemistry Analysis: 1:500 dilution of this antibody has been shown to detect Rhodopsin in human eye tissue.
ELISA: Specificity of this antibody, from a different lot, was validated in a competitive ELISA, as reported by Laird D, et al.
Quality
Evaluated by Immunohistochemistry in rat eye tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected Rhodopsin in rat eye tissue.
Target description
Expected at ~ 39 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Rat eye tissue
Rat eye tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Liang Jia et al.
The Journal of biological chemistry, 297(5), 101268-101268 (2021-10-04)
Biogenic amines activate G-protein-coupled receptors (GPCRs) in the central nervous system in vertebrate animals. Several biogenic amines, when excreted, stimulate trace amine-associated receptors (TAARs), a group of GPCRs in the main olfactory epithelium, and elicit innate behaviors. How TAARs recognize
Kayvan Samimi et al.
Biomedical optics express, 13(6), 3476-3492 (2022-07-06)
Photoreceptors are the key functional cell types responsible for the initiation of vision in the retina. Phototransduction involves isomerization and conversion of vitamin A compounds, known as retinoids, and their recycling through the visual cycle. We demonstrate a functional readout
Zhe Zhang et al.
The Journal of biological chemistry, 295(34), 12153-12166 (2020-07-09)
Pheromones play critical roles in habitat identification and reproductive behavior synchronization in the sea lamprey (Petromyzon marinus). The bile acid 3-keto petromyzonol sulfate (3kPZS) is a major component of the sex pheromone mixture from male sea lamprey that induces specific
Divya Sinha et al.
American journal of human genetics, 107(2), 278-292 (2020-07-25)
Dominantly inherited disorders are not typically considered to be therapeutic candidates for gene augmentation. Here, we utilized induced pluripotent stem cell-derived retinal pigment epithelium (iPSC-RPE) to test the potential of gene augmentation to treat Best disease, a dominant macular dystrophy
Ashley A Rowe et al.
JCI insight, 6(17) (2021-07-23)
The metabolic environment is important for neuronal cells, such as photoreceptors. When photoreceptors undergo degeneration, as occurs during retinitis pigmentosa (RP), patients have progressive loss of vision that proceeds to full blindness. Currently, there are no available treatments for the
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