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MABF63

Sigma-Aldrich

Anti-Activation-induced cytidine deaminase (AID) Antibody, clone 328.8

clone 328.8, from rat

Synonym(s):

activation-induced cytidine deaminase, integrated into Burkitt′s lymphoma cell line Ramos, Cytidine aminohydrolase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

328.8, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_065712

UniProt accession no.

Q9GZX7

shipped in

wet ice

target post-translational modification

unmodified

General description

Activation-induced cytidine deaminase (AID) is a 24 kDa, RNA-editing protein that is necessary for the initiation of both somatic hypermutation (SHM) and class switch recombination (CSR) of the Ig gene through the production of U:G mismatches in DNA, and is primarily found in activated B cells. AID expression has also been observed in several varying types of B cell lymphomas such as; Burkitt’s lymphoma, Hodgkin’s lymphoma, hairy cell lymphoma, follicular lymphoma, mantle cell lymphoma, mucosa-associated lymphoid tissue lymphoma, and mediastinal B cell lymphoma. Mutations in AID expression are causal to autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2), which is characterized by serum containing IgM but lacking IgG, IgA and IgE, which leads to severe susceptibility to bacterial infections.

Immunogen

KLH-conjugated linear peptide corresponding to human Activation-induced cytidine deaminase (AID).

Application

Anti-Activation-induced cytidine deaminase (AID) Antibody, clone 328.8 detects level of Activation-induced cytidine deaminase (AID) & has been published & validated for use in WB, IH.
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Matthew Scharff, Albert Einstein, matthew.scharff@einstein.yu.edu.)
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Activation-induced cytidine deaminase (AID) on 10 µg of Raji cell lysate.

Target description

~ 24 kDa observed. Uncharacterized bands may be observed in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Takashi Iizuka et al.
Scientific reports, 11(1), 3833-3833 (2021-02-17)
Activation-induced cytidine deaminase (AID, Aicda) is a master gene regulating class switching of immunoglobulin genes. In this study, we investigated the significance of AID expression in the ovary. Immunohistological study and RT-PCR showed that AID was expressed in murine granulosa

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