MABF556
Anti-CD279 (PD-1) Antibody (mouse), PE, clone J43.1
clone J43.1, 0.2 mg/mL, from hamster(Armenian)
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Programmed cell death protein 1, Protein PD-1, mPD-1, CD279
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biological source
hamster (Armenian)
Quality Level
conjugate
PE
antibody form
purified antibody
antibody product type
primary antibodies
clone
J43.1, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Pdcd1(18566)
Related Categories
General description
The J43.1 antibody is specific for mouse CD279, also known as programmed death-1 (PD-1), a 55 kDa glycoprotein which can co-regulate T cell antigen receptor signaling and therefore modulate T cell activation. PD-1 exists in a monomeric form that is expressed by CD4- CD8- thymocytes, where it participates in the processes of clonal selection, elimination of autoreactive lymphocytes, and development of tolerance. PD-1 expression is also inducible upon activation of mature T cells, where it has been proposed to interact with the co-stimulatory receptor CD80 to limit T cell activation. Two ligands for PD-1, known as PD-L1 (B7-H1) and PD-L2 (B7-DC) are differentially expressed on T and B cells, monocytes, macrophages, NK cells or dendritic cells. PD-1 is a member of a family of receptors including CD28 and CTLA-4 (CD152), which interact with “B7” ligands to provide a balance of co-stimulatory /co-inhibitory signaling important in T cell activation, tolerance, and autoimmunity.The J43.1 antibody may be used as a marker for PD-1 expression, and is commonly used for analysis of receptor-ligand interaction and function(s) in vitro and in vivo.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
This armenian hamster monoclonal antibody Anti-CD279 (PD-1) (mouse), PE, clone J43.1 validated for use in IP, IHC & Flow Cytometry for the detection of CD279 (PD-1).
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD279 (PD-1) in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.5 µg of this antibody detected CD279 (PD-1) in one million C57Bl/6 splenocytes.
Physical form
Purified Armenian Hamster Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Storage and Stability
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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