MABF550
Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5
clone PC61.5, 0.2 mg/mL, from rat
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Synonym(s):
Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55, CD25
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44
Recommended Products
biological source
rat
Quality Level
conjugate
PerCP-Cy5.5
antibody form
purified antibody
antibody product type
primary antibodies
clone
PC61.5, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1λ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Il2Ra(16184)
Related Categories
General description
The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
Application
This rat monoclonal antibody Anti-CD25 (mouse), PerCP-Cy5.5, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.25 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
WGK
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Certificates of Analysis (COA)
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