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MABF474

Sigma-Aldrich

Anti-Ly-6G (Gr-1) Antibody (mouse), clone RB6-8C5

clone RB6-8C5, 0.5 mg/mL, from rat

Synonym(s):

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

RB6-8C5, monoclonal

species reactivity

mouse

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable

isotype

IgG2bκ

UniProt accession no.

P35461

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

Related Categories

General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.

Specificity

M

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
This Rat Monoclonal Antibody Anti-Ly-6G (Gr-1) (mouse), clone RB6-8C5, Cat. No. MABF474 is validated for use in Immunohistochemistry, Flow Cytometry for the detection of Ly-6G (Gr-1).

Physical form

Format: Purified
Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Storage and Stability

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Anja A Kühl et al.
Gastroenterology, 133(6), 1882-1892 (2007-12-07)
Neutrophils are generally thought to play an important proinflammatory role in the pathogenesis of inflammatory bowel disease. The objective of this study was to evaluate whether blocking the invasion of neutrophils by anti-L-selectin monoclonal antibodies modulates chemically induced colitis and

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