Anti-CD3 (mouse) Antibody, Alexa Fluor^™647, clone 17A2

CD3, also known as T3, is a member of the Ig and the ITAM superfamilies. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon and zeta. CD3 is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The CD3 antigen is present in early thymocytes, and has been considered the best all-around T cell marker. The positive staining of this marker may represent a sign of early commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies. CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

This antibody recognizes ε/γ (but not ε/δ) of the CD3 complex.

γδTCR-positive T-T hybridoma D1

Anti-CD3 (mouse) Antibody, AlexaFluor647, clone 17A2 is a highly specific rat monoclonal antibody, that targets CD3 & has been tested in Flow Cytometry, IHC, Activity Assay & IP.

Immunohistochemistry Analysis: A previous lot detected human CD3 in Immunohistochemistry. (Zhang Y, et al. 2002; Miescher GC, et al. 1989.)

Immunoprecipitation Analysis: A previous lot detected human CD3 in Immunoprecipitation. (Miescher GC, et al. 1989.)

Activity Assay Analysis: A previous lot detected human CD3 in Activity Assay. (Miescher GC, et al. 1989.)

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The antibody was purified by affinity chromatography, and conjugated with AlexaFluor647 under optimal conditions. The solution is free of unconjugated AlexaFluor647 and unconjugated antibody. Alexa Fluor 647 is a registered trademark of Molecular Probes, Inc. Alexa Fluor 647 dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD3 in one million C57BL/6 mouse splenocytes.

21 kDa calculated

Affinity

Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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