MABF360
Anti-CD117 (c-kit) Antibody (mouse), APC, clone ACK2
clone ACK2, 0.2 mg/mL, from rat
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Mast/stem cell growth factor receptor Kit, SCFR, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117
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biological source
rat
Quality Level
conjugate
Allophycocyanin conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
ACK2, monoclonal
species reactivity
mouse
concentration
0.2 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Kit(16590)
Related Categories
General description
CD117, also called c-Kit, is a 145 kDa cytokine receptor important in the development of hematopoietic stem cells, in oogenesis, and for functional activity of immune cells such as NK and mast cells. c-Kit binds to a ligand known as stem cell factor (SCF), or alternatively as mast cell growth factor. Ligand binding promotes the activation (dimerization) and subsequent tyrosine kinase activity of the c-Kit receptor and triggers key survival, expansion and maturation signals during hematopoietic progenitor cell development. Conversely, shedding of extracellular domain of c-Kit receptor is reported to induce inactivation or apoptosis within these cells. The survival signaling activity of c-Kit confers a proto-oncogenic attribute to the receptor, as overexpression or mutations in this protein are associated with tumor development.
Specificity
This antibody is reported to be cross-reactive with rat CD117 and is extensively published for use in this species.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rat monoclonal antibody Anti-CD117 (c-kit) (mouse), APC & clone ACK2 validated for use in western blotting;IHC;ICC;Flow Cytometry for the detection of CD117 (c-kit).
Quality
The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 in one million C57BI/6 bone marrow cells.
Flow Cytometry Analysis: 0.06 µg of this antibody detected CD117 in one million C57BI/6 bone marrow cells.
Physical form
Purified rat monoclonal antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Size Exclusion Chromatography
Storage and Stability
It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
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Certificates of Analysis (COA)
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