Anti-LAP (TGF-β1) (mouse) Antibody, APC, clone TW7-16B4

LAP, also known as TGF-beta1 or TGFB1, belongs to the TGF-beta family. TGFB1 is a multifunctional protein that controls proliferation, differentiation and other functions in various cell types. TGFB1 is synthesized by many cells that have specific receptors for it. It positively and negatively regulates many other growth factors. LAP TGF-beta1 plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. The ′straitjacket′ and ′arm′ domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The physical force unfastens the straitjacket and releases the active growth factor dimer.

This antibody recognizes recombinant LAP, latent TGF-β, and pro-TGF-β.

Mouse Tgfb1-transduced P3U1 cells

Detect TGFβ using this mouse monoclonal antibody, Anti-LAP (TGF-β1) (mouse) Antibody, APC, clone TW7-16B4 validated for use in Flow Cytometry, western blotting, IP & Neutralizing.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Western Blotting Analysis: A representative lot detected human LAP (TGF-β1) in Western Blotting. (Oida T, et al. 2010.)

Immunoprecipitation Analysis: A representative lot detected human LAP (TGF-β1) in Immunoprecipitation. (Oida T, et al. 2010.)

Neutralization Assay Analysis: A representative lot detected human LAP (TGF-β1) in Neutralization Assay. (Oida T, et al. 2011.)

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

Flow Cytometry Analysis: 0.5 μg of this antibody detected LAP (TGF-β1) in one million CD3+CD28+IL-2-stimulated C57BL/6 mouse splenocytes.

44 kDa calculated

Affinity

Purified mouse conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.