MABF34
Anti-Thy-1 Antibody, clone M5/49
clone M549.4.1, from rat
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Synonym(s):
Thy-1 cell surface antigen, Thy-1 T-cell antigen, Thy-1 membrane glycoprotein, Thy-1 antigen, CD90 antigen
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M549.4.1, monoclonal
species reactivity
mouse
technique(s)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... THY1(7070)
General description
Thy-1 membrane glycoprotein (Thy-1) is a glycosylphosphatidylinositol (GPI)-anchored surface protein belonging to the immunoglobulin superfamily and is a major cell surface protein found on both T–cells and in the brain. Thy-1 is thought to be involved in interactions taking place during synaptogenesis and other neurological events between cells or cells and their ligands. There are two known alleles for Thy-1; Thy1.1 and Thy1.2. Studies have shown N-glycosolation to take place at three locations on the protein.
Immunogen
B6 Anti-BNL4Φ lymphocytes
Application
Anti-Thy-1 Antibody, clone M5/49 detects level of Thy-1 & has been published & validated for use in WB.
Functional Assay: A previous lot of this antibody was used for the inhibition of B6 Anti-BNLΦ CTL mediated cell death. (Davignon, D., et al. (1981). PNAS USA. 78(7):4535-4539.)
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Neural Stem Cells
Inflammation & Autoimmune Mechanisms
Neural Stem Cells
Quality
Evaluated by Western Blot in P388D1 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Thy-1 in 10 µg of P388D1 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Thy-1 in 10 µg of P388D1 cell lysate.
Target description
~22 kDa observed.
Higher bands may be observed due to glycosylation.
Higher bands may be observed due to glycosylation.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
P388D1 cell lysate
P388D1 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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