Anti-IL-10 (human) Antibody, PE, clone JES3-19F1

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) is a protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. IL-10 down regulates the expression of Th1 cytokines, major histocompatibility complex class II antigens, and costimulatory molecules on macrophages. It also enhaces B cell survival, proliferation, and antibody production. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. IL-10 is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. A polymorphism in IL10 promoter region is associated with resistance to graft-versus-host disease (GVHDS), a major complication of allogeneic bone marrow transplantation, in which mature donor T-cells that contaminate the allogeneic bone marrow recognize the tissues of the recipient as foreign, causing a severe inflammatory disease. Knockout studies suggest that IL-10 is an immunoregulator in the intestinal tract.

COS-expressed recombinant human IL-10

Anti-IL-10 (human) Antibody, PE, clone JES3-19F1 is a highly specific rat monoclonal antibody & that targets IL-10 & has been tested in Flow Cytometry.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 20 μL of this antibody detected IL-10 in one million LPS-stimulated human peripheral blood monocytes.

21 kDa calculated

Affinity

Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide and 0.2% (w/v) BSA.

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.