Anti-CD226/DNAM-1 Antibody, clone 10E5

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD226/DNAM-1 in C57BL/6 mouse splenocytes.

Tested Applications

Inhibition: A representative lot inhibited Th1 cell expansion and in the induction of APCs that inhibit T cell activation. (Dardalhon, V., et al. (2005). J Immunol. 175(3);1558-65).

Flow Cytometry Analysis: A representative lot detected CD226/DNAM-1 in Flow Cytometry applications (Dardalhon, V., et al. (2005). J Immunol. 175(3);1558-65; Fang, L., et al. (2009). J Immunol. 182(9):5453-60; Zhang, Z., et al. (2015). J Exp Med. 212(12):2165-82).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 10E5 is a rat monoclonal antibody that detects CD226/DNAM-1. It targets an epitope within the extracellular domain of the protein.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

CD226 antigen (UniProt: Q5DW69; also known as Dnam-1, DNAX accessory molecule-1) is encoded by the Cd226 gene (Gene ID: 225825; also known as Dnam-1) in mouse. CD226 is a cell surface glycoprotein that belongs to the immunoglobulin superfamily. It contains 2 Ig-like C2-type domains (aa 19-126 and 297-316) and a transmembrane domain (aa 255-275). It is primarily expressed on the surface of natural killer (NK) cells, T cells, monocytes, and platelets. CD226 plays a crucial role in the regulation of immune responses, particularly in the context of cell-mediated cytotoxicity and immune surveillance. CD226 is involved in the modulation of T cell responses and the regulation of immune tolerance. Dysregulation of CD226 has been implicated in various autoimmune diseases, lupus and cancer, making it an important target for immunotherapy research and treatment development. (Ref.: Huang, Z., et al., (2020). Front. Cell Dev. Biol. 24; Article 564).

Th1-polarized T cell clones (AE7).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Purified rat monoclonal antibody IgG2b in PBS without azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.