Anti-IL-16 Antibody, clone 14.1

Originally known as lymphocyte chemoattractant factor (LCF), Interleukin-16 (IL-16) is a product of human peripheral mononuclear cells when antigen-stimulated or mitogen-stimulated. It is also produced by dendritic cells, epithelial cells, eosinophils, fibroblasts and mast cells. The pro-form of IL-16 is a 631 amino acid protein that is cleaved by caspase 3 in activated CD4+ T cells where it is then secreted and is accessible for binding CD4 receptors. IL-16 regulates and induces migratory response in CD4+ lymphocytes, eosinophils, and monocytes. It is a soluble ligand for CD4 and functions to prime responsiveness to IL-2 and IL-15 in CD4+ T-cells. Although Isoform 1’s function is thought to be as an anchor to membrane ion channels, Isoform 3 is more widely researched and characterized as component of T-cell cycle progression.

Epitope: Unknown

GST-tagged recombinant protein corresponding to the C-terminal region of secreted human IL-16.

Detect IL-16 using this Anti-IL-16 Antibody, clone 14.1 validated for use in WB, NEUT, IH, IC, FC.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH to detect IL-16 in naive kidney tissue (Wang, S., et al., 2008).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC to detect IL-16 in spinal cord cells (Skundric, D., et al., 2005).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC to detect IL-16 in human PBMCs (McFadden, C., et al., 2007).

Neutralizing Activity Assay: 1–10 µg/mL of a previous lot was used by an independent laboratory at concentrations sufficient to neutralize bioactivity of IL-16 (Morgan, R., et al., 2007).

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Evaluated by Western Blot in human serum.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected IL-16 in 10 µg of human serum.

~20 kDa observed.
Monomers of IL-16 migrate on SDS-PAGE in the range of 17–20 kDa (Zhang, Y., et al., 1998). Active IL-16 (clone 14.1) is detected at ~17 kDa in WB (Skundric, D., et al., 2005).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Human serum

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.