Anti-STING Antibody, clone S17G2C4B7

Stimulator of interferon genes protein (UniProt Q86WV6; also known as Endoplasmic reticulum interferon stimulator, ERIS, hMITA, hSTING, Mediator of IRF3 activation, Transmembrane protein 173) is encoded by the TMEM173 (also known as ERIS, MITA, MPYS, SAVI, STING) gene (Gene ID 340061) in human. STING is an adaptor protein activated by cytosolic dsDNA or cyclic dinucleotides and plays a critical role in initiating innate responses to infection by various pathogens, including Herpes simplex virus (HSV). Once activated, STING translocates to the endoplasmic reticulum and activates the TBK1/IRF3 pathway that in turn mediates the upregulation of interferon (IFN) β. In addition, the DNA sensor IFI16 is also known to function as a STING ligand and potentiates STING-dependent sensing of viral infection by presenting cellular viral DNA to STING. STING is a four transmembrane (a.a. 21-41, 47-67, 87-106, 116-136) protein with both its N- and C-terminal ends exposed intracellularly (a.a. 1-20 and 137-379).The C-terminal cytoplasmic tail contains the c-di-GMP-binding domain (CBD; a.a. 153-340) with two c-di-GMP-binding regions (a.a. 162-167 & 238-241).

Clone S17G2C4B7 recognizes an epitope in the C-terminal cytoplasmic domain of human and murine STING.

Epitope: Amino acids 301-315.

His-tagged recombinant human STING C-terminal cytoplasmic domain fragment.

Anti-STING Antibody, clone S17G2C4B7 is an antibody against STING for use in Western Blotting, Immunocytochemistry.

Research Category
Inflammation & Immunology

Research Sub Category
Infectious Diseases - Viral

Western Blotting Analysis: A 1:200 dilution from a representative lot detected the expression of exogenously transfected human and murine STING in HEK293T cells, as well as the endogenous STING in telomerase-immortalized human foreskin fibroblasts hTERT-BJ1 (Courtesy of Dr. Glen N. Barber, University of Miami School of Medicine, FL, U.S.A.).
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected the expression of exogenously transfected human and murine STING in HEK293T cells, as well as the endogenous STING in telomerase-immortalized human foreskin fibroblasts hTERT-BJ1 (Courtesy of Dr. Glen N. Barber, University of Miami School of Medicine, FL, U.S.A.).

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected STING in 50 µg of HEK293T cell lysate.

~38 kDa observed. 42.19 kDa calculated.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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