MABF235
Anti-AIRE Antibody, clone Mab155
clone Mab155, 1 mg/mL, from mouse
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Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED protein
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Mab155, monoclonal
species reactivity
human
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AIRE(326)
Related Categories
General description
AIRE (Autoimmune Regulator) is a human gene that is expressed primarily in the thymus. It causes transcription of a wide selection of organ-specific genes, reducing the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells via negative selection. Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendo-crinopathy-candidiasis-ectodermal dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. Aire functions through initiating the transcription of a diverse set of self-antigens, such as insulin, in the thymus. This expression then allows maturing thymocytes to become tolerant towards peripheral organs, thereby suppressing autoimmune disease. Aire is atranscriptional regulator protein that binds to DNA as dimer and tetramer, but not as a monomer. It forms nuclear bodies and interacts with the transcriptional coactivator CBP (CREBBP). At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD).
Immunogen
His-tagged recombinant protein corresponding to human AIRE.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Immunological Signaling
This Anti-AIRE Antibody, clone Mab155 is validated for use in Flow Cytometry and Western Blotting and Immunocytochemistry for the detection of AIRE.
Western Blotting Analysis: A representative lot detected AIRE in Cos1 transfected cell lysate (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Immunocytochemistry Analysis: A representative lot detected AIRE in freshly isolated endogenous peripheral blood lymphocytes (Rinderle, C., et al. (1999). Human Molecular Genetics. 8(2):277-290).
Quality
Evaluated by Flow Cytometry in human PBMC cells.
Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.
Flow Cytometry Analysis: 0.5 µg of this antibody detected AIRE in human PBMC cells.
Target description
58 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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