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antibody form
purified antibody
clone
2t2
monoclonal
purified by
using protein G
species reactivity
virus, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
General description
A linear peptide corresponding to 17 amino acids from the J-domain region present in both MCV LT and sT proteins.
Specificity
Clone 2t2 is a mouse monoclonal antibody that detects MCV LT and the 57 kDa T.
Application
Immunohistochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunohistochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
Quality
Evaluated by Western Blotting in MKL-1 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.
Physical properties
~17 and 48 kDa observed; 28.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Virus transformed human cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulatory Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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