Anti-MIC-A MIC-B Antibody, clone 6D4

MHC class I polypeptide-related sequence A/MHC class I polypeptide-related sequence B (UniProt: Q29983/Q29980; also known as MIC-A/MIC-B) is encoded by the MICA/MICB (also known as PERB11.1/PERB11.2) gene (Gene ID: 100507436/4277) in human. MIC-A and B are closely related single-pass type I membrane proteins that act as stress-induced self-antigen that are recognized by gamma-delta T cells and serve as ligands for the KLRK1/NKG2D receptor. They are recognized by cytotoxic gamma-delta T cells expressing the TCR variable region V delta1. Under normal conditions expression of MIC-A and B is restricted mainly to the intestinal epithelium. However, their expression has been reported in tumor cells. They are proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. MIC-A and B can be induced by heat shock and by exposure to DNA damaging conditions. They are synthesized with a signal peptide (aa 1-23 and 1-22, respectively for MIC-A and MIC-B), which are cleaved off in the mature form. Their mature forms contain an extracellular domain (aa 24-304 and 23-309), a transmembrane domain (aa 308-328 and 310-330), and a cytoplasmic domain (aa 329-383 and 331-383), respectively. (Ref.: Groh, V., et al. (1999). Proc. Natl. Acad. Sci USA. 96 (12); 6879-6884).

Clone 6D4 specifically stains MIC-A and MIC-B in human cells.

C1R cells expressing MICA.

Anti-MIC-A/MIC-B, Clone 6D4, Cat. No. MABF2160, is a mouse monoclonal antibody that detects MHC class I polypeptide-related sequence A/MHC class I polypeptide-related sequence B (MIC-A and MIC-B) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Activity/

Immunohistochemistry (Paraffin) Analysis: A representative lot detected MIC-A and MIC-B in Immunohistochemistry applications (Groh, V., et. al. (1999). Proc Natl Acad Sci U S A. 96(12):6879-84).

Flow Cytometry Analysis: A representative lot detected MIC-A and MIC-B in Flow Cytometry applications (Groh, V., et. al. (1999). Proc Natl Acad Sci U S A. 96(12):6879-84).

Inhibits Activity/Function Analysis: A representative lot inhibited cytotoxicity of ovary tumor-derived T cells against various cancer cell lines. (Groh, V., et. al. (1999). Proc Natl Acad Sci U S A. 96(12):6879-84).

Research Category
Inflammation & Immunology

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 µg of this antibody detected MIC-A MIC-B in one million HeLa cells.

42.91 and 42.64 kDa calculated, respectively for MIC-A and MIC-B.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.