MABF2105
Anti-CD6 Antibody, clone UMCD6
clone UMCD6, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(1)
T-cell differentiation antigen CD6, T12, TP120
Recommended Products
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
UMCD6, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
technique(s)
dot blot: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
inhibition assay: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... CD6(923)
Related Categories
General description
T-cell differentiation antigen CD6 (UniProt: P30203; also known as T12, TP120, CD6) is encoded by the CD6 gene (Gene ID: 923) in human. CD6 is a single-pass type I membrane protein that serves as a cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166. It contributes to signaling cascades triggered by activation of the TCR/CD3 complex and functions as a costimulatory molecule to promote T-cell activation and proliferation. It is also involved in the formation and maturation of the immunological synapse. CD6 has been detected on peripheral blood T-cells and on natural killer (NK) cells and is also detected in spleen, thymus, appendix, lymph nodes. CD6 is known to bind both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria and LPS binding leads to the activation of signaling cascades and down-stream MAP kinases. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 18-402), a transmembrane domain (aa 403-423), and a cytoplasmic domain (aa 424-668). Seven isoforms of CD6 have been reported that are produced by alternative splicing.
Specificity
Clone UMCD6 specifically detects CD6 in human cells.
Immunogen
ST-1 T cells isolated from human knee synovium.
Application
Anti-CD6, clone UMCD6, Cat. No. MABF2105 is a mouse monoclonal antibody that detects CD6 and has been tested for use in Dot Blot, Flow Cytometry, Immunoprecipitation, Function Analysis, Inhibition assay.
Inhibition Analysis: A representative lot inhibited antigen-specific responses to tetanus toxoid, but not to the superantigen SEA. (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43).
Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Dot Blot Analysis: A representative lot detected CD6 in Dot Blot applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. al. (1994). J Immunol. 153(1):1-9; Li, Y., et. al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692).
Induces Function: A representative lot showed strong synergistic effect with phorbol ester in inducing T cell activation and enhanced the autologous mixed lymphocyte reaction. (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Dot Blot Analysis: A representative lot detected CD6 in Dot Blot applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD6 in Immunoprecipitation applications (Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92).
Flow Cytometry Analysis: A representative lot detected CD6 in Immunoprecipitation applications (Singer, N.G., et. al. (1996). Immunology. 88(4):537-43; Bott, C.M., et. al. (1993). Int Immunol. 5(7):783-92; Bott, C.M., et. al. (1994). J Immunol. 153(1):1-9; Li, Y., et. al. (2017). Proc Natl Acad Sci USA. 114(10):2687-2692).
Quality
Evaluated by Flow Cytometry in PMA stimulated Jurkat cells.
Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA.
Flow Cytometry Analysis: A 1:1000 dilution of this antibody detected CD6 in one million Jurkat cells stimulated with PMA.
Target description
71.80 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
Regulatory Information
新产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service