MABF2089
Anti-CD28 Antibody, clone 37.51
clone 37.51, from hamster(Syrian)
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T-cell-specific surface glycoprotein CD28
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biological source
hamster (Syrian)
antibody form
purified antibody
antibody product type
primary antibodies
clone
37.51, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Cd28(12487)
Related Categories
General description
T-cell-specific surface glycoprotein CD28 (UniProt: P31041, also known as CD28) is encoded by the Cd28 gene (Gene ID: 12487) in murine species. CD28 is a single-pass type I membrane protein that exists as a disulfide linked homodimer. In human CD28 is expressed by a majority of T cells in the periphery, including all CD4+ cells and about 50% CD8+ cells. CD28 is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. It has an extracellular domain (aa 2-150), a transmembrane domain (aa 151-177), and a cytoplasmic domain (aa 178-218). CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. It enhances the production of IL-4 and IL-10 in T-cells in conjunction with TCR/CD3 ligation and CD40L co-stimulation. B7-1 (CD80) and B7-2 (CD86) are two known ligands of CD28 on activated B cells and antigen presenting cells (APC). These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction co-stimulatory signaling between the B7 ligands and CD28 is essential for crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
Specificity
Clone 37.51 is a Syrian hamster monoclonal antibody that detects CD28 in murine cells.
Immunogen
EL-4 T lymphoma cells.
Application
Affects Function Analysis: A representative lot detected CD28 in Affects Function applications (Ulges, A., et. al. (2015). Nat Immunol. 16(3):267-75; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65; Brender, C., et. al. (2007) Blood. 1;110(7):2528-36).
Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).
Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).
Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8
Flow Cytometry Analysis: A representative lot detected CD28 in Flow Cytometry applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8; Krummel, M.F., et. al. (1995). J Exp Med. 1;182(2):459-65).
Immunoprecipitation Analysis: A representative lot detected CD28 in Immunoprecipitation applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8).
Western Blotting Analysis: A representative lot detected CD28 in Western Blotting applications (Gross, J.A., et. al. (1992). J Immunol. 149(2):380-8
This syrian hamster monoclonal Anti-CD28, clone 37.51 Antibody, Cat. No. MABF2089 is tested for use in Flow Cytometry, Western Blotting, Immunoprecipitation, and Affects Function, for the detection of CD28.
Quality
Evaluated by Flow Cytometry in EL-4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD28 in 1X10E6 EL-4 T lymphoma cells.
Target description
25.24 kDa calculated.
Physical form
Format: Purified
Purified syrian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Other Notes
Concentration: Please refer to lot specific datasheet.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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