MABF1971M
Anti-NKR-P1B (CD161b) Antibody, clone Stok27
clone Stok27, from rat
Sign Into View Organizational & Contract Pricing
All Photos(1)
Synonym(s):
Killer cell lectin-like receptor subfamily B member 1B allele B, CD161 antigen-like family member B, Immunoreceptor NKR-P1C, Natural killer cell surface protein NKR-P1B allele WAG/PVG/BS, Natural killer lymphocyte receptor P1B, CD161b
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
Stok27, monoclonal
species reactivity
rat
packaging
antibody small pack of 25 μL
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
rat ... Klrb1C(683758)
Related Categories
General description
Killer cell lectin-like receptor subfamily B member 1B allele B (UniProt: Q5NKN4; also known as CD161 antigen-like family member B, Immunoreceptor NKR-P1C, Natural killer cell surface protein NKR-P1B allele WAG/PVG/BS, Natural killer lymphocyte receptor P1B, CD161b) is encoded by the Klrb1b (also known as Nkrp1b, Nkrp1c) gene (Gene ID: 678513) in rat. NKR-P1B is a single-pass type II membrane protein that is expressed in a subset of natural killer cells. It consists of a cytoplasmic domain (aa 1-45), a helical domain (aa 46-66), and an extracellular domain (aa 67-223). In contrast to NKR-P1C, which functions as an activating receptor, NKR-P1B inhibits NK cell activation. NKR-P1b serves as a receptor for CLEC2D/OCIL and ligand binding contributes to inhibition of cytotoxic natural killer cells. It may also mediate MHC class I-independent ′missing-self′ recognition of allografts, tumor cells, and virus-infected cells. The inhibitory NKR-P1B receptor identifies rat splenic NK cells that are low in Ly49 receptors, but are enriched in CD94/NKG2 receptors. (Ref.: Inngjerdingen, M et al. (2012). J. Immunol. 188(6); 2499-2508; Carlyle, JR et al. (1999). J. Immunol. 162(10); 5917-5923).
Specificity
Clone Stok27 is a rat monoclonal antibody that detects Killer cell lectin-like receptor subfamily B member 1B allele B (NKR-P1B) in rat. It targets an epitope within the extracellular domain.
Immunogen
IL-2-activated NK cells from PVG.1N (RT1n) rats.
Application
Anti-NKR-P1B (CD161b), clone Stok27, Cat. No. MABF1971, is a rat monoclonal antibody that detects NKR-P1B and has been tested for use in Flow Cytometry and Immunoprecipitation.
Immunoprecipitation Analysis: A representative lot detected NKR-P1B (CD161b) in Immunoprecipitation applications (Kveberg, L., et. al. (2006). J Immunol. 176(7):4133-40).
Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Kveberg, L., et. al. (2006). J Immunol. 176(7):4133-40; Smelt, M.J., et. al. (2014). Cell Transplant. 23(11):1381-94).
Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Kveberg, L., et. al. (2006). J Immunol. 176(7):4133-40; Smelt, M.J., et. al. (2014). Cell Transplant. 23(11):1381-94).
Quality
Evaluated by Flow Cytometry in Rnk16 (Rat NK) cells stably transfected with Stok27 (NKR-1B) antigen.
Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1B (CD161b) in Rnk16 (Rat NK) cells stably transfected with Stok27 (NKR-1B) antigen.
Flow Cytometry Analysis: 1 µg of this antibody detected NKR-P1B (CD161b) in Rnk16 (Rat NK) cells stably transfected with Stok27 (NKR-1B) antigen.
Target description
24.88 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service