MABF1968
Anti-Microglobulin beta2 Antibody, clone BBM.1
clone BBM.1, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(2)
B2M
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BBM.1, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
inhibition assay: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... B2M(567)
Related Categories
General description
Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.
Specificity
Clone BBM.1 detects Beta-2-microglobulin in human.
Immunogen
MOLT-4, a Human acute lymphoblastic leukemia cell line.
Application
Anti-Microglobulin beta2, clone BBM.1, Cat. No. MABF1968, is a mouse monoclonal antibody that detects Microglobulin beta2 and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assays, and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).
Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).
Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).
Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.
Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).
Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).
Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Quality
Evaluated by Western Blotting in MOLT-4 cell lysates
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.
Target description
~14 kDa observed; 13.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service