MABF161A
Anti-CD45 (human), violetFluor® 450, clone HI30 Antibody
clone HI30, from mouse, violetFluor® 450
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Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45
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biological source
mouse
Quality Level
conjugate
violetFluor® 450
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
HI30, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... PTPRC(5788)
Related Categories
General description
The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.
Application
This mouse monoclonal Anti-CD45 Antibody (human), violetFluor® 450, clone HI30, Cat. No. MABF1611 is validated for use in Flow Cytometry, for the detection of CD45.
Quality
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.
Flow Cytometry Analysis: 1 μg from a representative lot detected CD45 in one million human peripheral blood lymphocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
violetFluor is a registered trademark of Tonbo Biotechnologies
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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