MABF157E
Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody
clone 145-2C11, from hamster(Armenian), purified by affinity chromatography
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Synonym(s):
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3e
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45
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biological source
hamster (Armenian)
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
145-2C11, monoclonal
purified by
affinity chromatography
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
flow cytometry: suitable
isotype
IgG
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Cd3E(12501)
Related Categories
General description
The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The 145-2C11 antibody is a widely used phenotypic marker for mouse T cells. In addition, binding of 145-2C11 antibody to CD3e can induce cell activation. A recent publication of the crystal structure of a murine CD3e-mitogenic antibody complex provides further insight into the action of commonly used agonist antibodies (Fernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335).
Application
Anti-CD3e (Mouse), clone 145-2C11, biotin conjugate Antibody detects level of CD3e and has been published and validated for use in Flow Cytometry.
Quality
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD3e in one million C57Bl/6 splenocytes.
Other Notes
Concentration: Please refer to lot specific datasheet.
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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