MABE952
Anti-Histone H3.1 Antibody, clone 1D4F2
clone 1D4F2, 1 mg/mL, from rat
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Synonym(s):
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1D4F2, monoclonal
species reactivity
human, mouse
concentration
1 mg/mL
technique(s)
ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Immunogen
KLH-conjugated linear peptide corresponding to human Histone H3.1.
Application
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Histone H3.1 in HeLa cells.
Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone H3.1 in NIH/3T3 cells (Prof. Taro Tachibana, Cell Engineering Corporation.).
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected Histone H3.1 in C2C12 cells (Harada, A., et al. (2012). EMBO J. 31(13):2994-3007.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
Chromatin Immunoprecipitation Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3.1 (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
This Anti-Histone H3.1 Antibody, clone 1D4F2 is validated for use in western blotting, ICC, IP, ChIP & ChIP-seq for the detection of Histone H3.1.
Quality
Evaluated by Western Blotting in HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.
Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.1 in 10 µg of HeLa acid extract.
Target description
~17 kDa observed
Physical form
Format: Purified
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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