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MABE917

Sigma-Aldrich

Anti-DNA G-quadruplex structures Antibody, clone BG4

clone BG4, from Escherichia coli

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Synonym(s):
DNA G-quadruplex (BG4)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

Escherichia coli

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

BG4, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

ChIP: suitable (ChIP-seq)
ELISA: suitable
immunocytochemistry: suitable

shipped in

ambient

target post-translational modification

unmodified

General description

DNA G-quadruplex structures are associated with a number of important aspects of genome function, which include transcription, recombination and replication. Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4), which can influence methylation at CpG islands (CGIs). G4 structure is reported to be tightly associated with CGI hypomethylation in the human genome. Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an attractive topic in molecular biomedical research.(Ref.: Mao, SQ et al., (2018). Nat. Struc. Mol. Biol. 25(10); 951-957).

Specificity

Clone BG4 has a high affinity for intramolecular and intermolecular DNA G-quadruplexes with no detectable binding to a RNA hairpin, single-stranded DNA or double-stranded DNA.

Immunogen

G-quadruplex oligonucleotides.

Application

Anti-DNA G-quadruplex structures, clone BG4 Antibody, Cat. No. MABE917, is a highly specific monoclonal antibody that targets DNA G-quadruplex structures and has been tested in and ELISA, ChIP-seq, and Immunocytochemistry.
ELISA Analysis: A representative lot detected DNA G-quadruplex structures in Anti-DNA G-quarduplex structures, BG4 (Courtesy of University of Cambridge).

ChIP-seq Analysis: A representative lot detected DNA G-quadruplex structures in ChIP-seq applications (Hansel-Hertsch, R., et. al. (2016). Nat Genet. 48(10):1267-72).

ELISA Analysis: A representative lot detected DNA G-quadruplex structures in ELISA applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).

Immunocytochemistry Analysis: A representative lot detected DNA G-quadruplex structures in Immunocytochemistry applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).

Quality

The Anti-DNA-G-quadruplex structures, clone BG4 antibody (Cat. No. MABE917) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with Anti-FLAG epitope tag antibody (MAB3118).

Physical form

Format: Purified
Purified monoclonal antibody in PBS with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Keisuke Iida et al.
Scientific reports, 12(1), 12892-12892 (2022-07-29)
G-quadruplexes (G4s) regulate various biological processes in cells. However, cellular imaging of dynamically forming G4s in biomolecular condensates using small molecules has been poorly investigated. Herein, we present a fluorescent light-up probe with the ability to selectively stabilize G4s and
Lijun Xiang et al.
Nucleic acids research, 50(12), 6953-6967 (2022-06-25)
G-quadruplex structure (G4) is a type of DNA secondary structure that widely exists in the genomes of many organisms. G4s are believed to participate in multiple biological processes. Acyl-CoA binding protein (ACBP), a ubiquitously expressed and highly conserved protein in
Katharina Schlag et al.
The FEBS journal, 287(20), 4481-4499 (2020-02-26)
5-Lipoxygenase (5-LO) is the initial enzyme in the biosynthesis of leukotrienes, which are mediators involved in pathophysiological conditions such as asthma and certain cancer types. Knowledge of proteins involved in 5-LO pathway regulation, including gene regulatory proteins, is needed to
Diwei Ho et al.
Nature nanotechnology, 13(12), 1148-1153 (2018-10-10)
Gold nanorods are one of the most widely explored inorganic materials in nanomedicine for diagnostics, therapeutics and sensing1. It has been shown that gold nanorods are not cytotoxic and localize within cytoplasmic vesicles following endocytosis, with no nuclear localization2,3, but
Zi-Fu Wang et al.
Cell chemical biology, 26(2), 179-190 (2018-12-06)
The most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) is an expanded G4C2 repeat [(G4C2)exp] in C9ORF72. ALS/FTD-associated toxicity has been traced to the RNA transcribed from the repeat expansion [r(G4C2)exp], which sequesters RNA-binding proteins
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