MABE912
Anti-Ikaros Antibody, clone 4E9
clone 4E9, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
DNA-binding protein Ikaros, Ikaros family zinc finger protein 1, Lymphoid transcription factor LyF-1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
4E9, monoclonal
species reactivity
human, mouse
technique(s)
ChIP: suitable (ChIP-seq)
flow cytometry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IKZF1(10320)
mouse ... Ikzf1(22778)
Related Categories
General description
Ikaros, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. Ikaros has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.
Immunogen
Recombinant protein corresponding to Human Ikaros.
Application
Flow Cytometry Analsysis: 1 µg of a representative lot of this antibody detected Ikaros in Mouse thymocytes
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).
ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).
Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP. See Zhang et al. (2012).
ChIP-seq Analsysis: A representative lot of this antibody as part of a blend Ikaros clones E9 and Ik14 (catalogue number MABE912 and MABE913 respectively) was demonstrated to perform in ChIP-seq. See Zhang et al. (2012).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Chromatin Biology
Transcription Factors
Chromatin Biology
This Anti-Ikaros Antibody, clone 4E9 is validated for use in Western Blotting and Flow Cytometry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Ikaros.
Quality
Evaluated by Western Blotting in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Ikaros in a Jurkat cell lysate.
Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Ikaros in a Jurkat cell lysate.
Target description
~60 kDa observed. Uncharacterized bands may be observed in some lysates.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Jingjie Chang et al.
Frontiers in immunology, 14, 1239779-1239779 (2023-09-04)
AIOLOS, encoded by IKZF3, is a member of the IKZF family of proteins that plays an important role in regulating late B-cell differentiation. Human individuals heterozygous for the AIOLOS p.N160S variant displayed impaired humoral immune responses as well as impaired
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service