MABE78
Anti-MiTF Antibody, clone 3D1.2
clone 3D1.2, from mouse
Synonym(s):
Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3D1.2, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MITF(4286)
Related Categories
General description
Microphthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Immunogen
GST-tagged recombinant protein corresponding to human MiTF.
Application
Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
Research Category
Signaling
Signaling
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
Target description
~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
Linkage
Replaces: MAB3747
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% sodium azide, pH 7.1.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shiyao Pei et al.
Experimental dermatology, 27(10), 1120-1125 (2018-07-26)
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