MABE344
Anti-BICC1/Protein Bicaudal C Homolog 1 Antibody, clone 2D9.1
clone 2D9.1, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(3)
Protein bicaudal C homolog 1, Bic-C
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2D9.1, monoclonal
species reactivity
human, mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BICC1(80114)
General description
Protein bicaudal C homolog 1 (UniProt Q9H694; also known as Bic-C) is encoded by the BICC1 (also known as CYSRD) gene (Gene ID 80114) in human. Drosophila bicaudal C and its mammalian homologs are RNA-binding proteins involved in symmetric distribution and localized translation of mRNAs of many proteins in a wide range of cells and tissues. RNA binding of Bic-C is mediated by N-terminal KH domains (a.a. 132-199 & 284-348), whereas a C-terminal sterile alpha motif (SAM; a.a. 873-936) self-polymerizes and localizes Bic-C at cytoplasmic foci where silencing of bound mRNAs takes place. Defective polymerization results in decreased Bic-C stability and thereby attenuates inhibition of Dishevelled-2 of Wnt/β-catenin pathway. Loss of BICC1 provokes renal and pancreatic cysts as well as ectopic Wnt/β-catenin signaling during visceral left-right patterning due to defective silencing of Bic-C target mRNAs, including adenylate cyclase 6 (AC6).
Specificity
Clone 2D9.1 reacts with both spliced isoforms of human and murine BICC1 reported by UniProt (Q9H694 & Q99MQ1).
Immunogen
Epitope: N-terminal half.
Recombinant fragment corresponding to the N-terminal half of human BICC1/Protein Bicaudal C Homolog 1.
Application
Anti-BICC1 Antibody, clone 2D9.1 is an antibody against Protein Bicaudal C Homolog 1 for use in Western Blotting, Immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Western Blotting Analysis: 1 µg/mL from a representative lot detected Bic-C/BICC1 in human HK and murine mIMCD-3 kidney cell lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY)
Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY).
Immunoprecipitation Analysis: 9 µg from a representative lot immunoprecipitated exogenously expressed human BICC1 isoforms 1 and 2 from 0.5 mL (~50-100 mg) of HEK293 transfectants lysates (Courtesy of Dr. Thomas Tuschl laboratory, The Rockefeller University, New York, NY).
Quality
Evaluated by Western Blotting of recombinant human BICC1.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected 10 µg of recombinant human BICC1.
Target description
~130 kDa observed. 104.8/105.0 kDa (Human/mouse isoform 1) and 94.29/93.41 kDa (Human/mouse isoform 2) calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service