MABE343-AF488
Anti-Puromycin, clone 12D10, Alexa Fluor™ 488 Conjugate Antibody
clone 12D10, 0.5 mg/mL, from mouse
Synonym(s):
Alexa Fluor 488 antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Puromycin, clone 12D10, Alexa Fluor™ 488 Conjugate Antibody, clone 12D10, 0.5 mg/mL, from mouse
Quality Level
biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
12D10, monoclonal
species reactivity
human
concentration
0.5 mg/mL
technique(s)
immunocytochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Analysis Note
Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor™ is a registered trademark of Life Technologies.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Biochem/physiol Actions
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
This antibody detects Puromycin-incorporated neosynthesized proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE343.
This product is a conjugated version of Cat. No. MABE343.
Refer to Cat. No. MABE343 for observed molecular weight information.
Immunogen
Puromycin-incorporated neosynthesized proteins at multiple molecular weights
Physical form
Protein G Purified
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Qi Wu et al.
Oxidative medicine and cellular longevity, 2019, 7064319-7064319 (2019-06-19)
Ribosome biogenesis is a crucial biological process related to cell proliferation, redox balance, and muscle contractility. Aortic smooth muscle cells (ASMCs) show inhibition of proliferation and apoptosis, along with high levels of oxidative stress in aortic dissection (AD). Theoretically, ribosome
Benjamin J Jenkins et al.
Cell metabolism, 35(7), 1132-1146 (2023-05-26)
Augmented T cell function leading to host damage in autoimmunity is supported by metabolic dysregulation, making targeting immunometabolism an attractive therapeutic avenue. Canagliflozin, a type 2 diabetes drug, is a sodium glucose co-transporter 2 (SGLT2) inhibitor with known off-target effects on
Danielle Minichino et al.
Journal of leukocyte biology, 112(5), 1089-1104 (2022-06-02)
Langerhans cell histiocytosis (LCH) is an inflammatory disease characterized by abnormal dendritic cells (DCs) with hyperactive ERK signaling, called "LCH cells." Since DCs rely on ERK signaling to produce inflammatory molecules in response to pathogenic cues, we hypothesized that hyperactive
Fangyuan Jia et al.
Oxidative medicine and cellular longevity, 2021, 5986260-5986260 (2021-01-30)
The rate of ribosome biogenesis plays a vital role in cell cycle progression and proliferation and is strongly connected with coronary restenosis and atherosclerosis. Blocking of proliferation 1 (BOP1) has been found as an evolutionarily conserved gene and a pivotal
Honyin Chiu et al.
iScience, 24(7), 102748-102748 (2021-07-20)
The cap-binding protein eukaryotic initiation factor 4E (eIF4E) promotes translation of mRNAs associated with proliferation and survival and is an attractive target for cancer therapeutics. Here, we used Eif4e germline and conditional knockout models to assess the impact of reduced
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