MABE284
Anti-MSH2 Antibody, clone FE11
clone FE11, from mouse
Synonym(s):
DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
FE11, monoclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
human
Citations:
23
Technique(s):
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
Uniprot accession no.:
Product Name
Anti-MSH2 Antibody, clone FE11, clone FE11, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FE11, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MSH2(4436)
Analysis Note
Control
SW-480 cell lysate
SW-480 cell lysate
Evaluated by Western Blot in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MSH2 in 10 µg of SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected MSH2 in 10 µg of SW-480 cell lysate.
Application
Anti-MSH2 Antibody, clone FE11 is a Mouse Monoclonal Antibody for detection of MSH2 also known as DNA mismatch repair protein Msh2, hMSH2, MutS protein homolog 2 & has been validated in WB & IHC.
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected SW-480 in human normal colonic tissue and in human adenocarcinoma tissue (Thibodeau, S. N., et al. (1996). 56(21):4836-4840.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
This antibody recognizes the C-terminus of MSH2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MSH2 is a ubiquitously expressed nuclear protein that is involved in DNA repair processes. MSH2 is active as a heterodimer of MutS alpha and beta subunits that bind DNA at mismatched strands. The MutS alpha subunit binds to single base mismatches and dinucleotide insertion-deletion loops, whereas MutS beta detects longer insertion-deletion loops. Bound MutS subunits then form complexes with MutL alpha dimers, to coordinate downstream DNA-mismatch–repair processes. Abnormal expression of MSH2 has been linked to hereditary non-polyposis colorectal cancer type 1 and Muir-Torre syndrome.
~104 kDa observed
Immunogen
Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human MSH2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Y Parc et al.
Gut, 53(3), 371-375 (2004-02-13)
Microsatellite instability (MSI) has been identified as a factor with good prognosis and chemosensitivity in stage III C colon cancer. The purpose of this study was to evaluate the routine use of immunohistochemical analysis (immunohistochemical staining of MSH2 and MLH1)
Anni Niskakoski et al.
International journal of cancer, 133(11), 2596-2608 (2013-05-30)
Ovarian carcinoma in Lynch syndrome (LS) is associated with unexpectedly high survival; yet, beyond DNA mismatch repair (MMR) defects, the developmental mechanisms are unknown. We used established (genetic) and new (epigenetic) classifiers of ovarian cancer to explore similarities and differences
V S Warneke et al.
British journal of cancer, 109(8), 2217-2227 (2013-09-07)
We investigated the expression of members of the epithelial cell adhesion molecule (EpCAM) signalling pathway in gastric cancer (GC) testing the following hypotheses: are these molecules expressed in GC and are they putatively involved in GC biology. The study cohort
Stéphanie Tomé et al.
PLoS genetics, 9(2), e1003280-e1003280 (2013-03-08)
Expansions of trinucleotide CAG/CTG repeats in somatic tissues are thought to contribute to ongoing disease progression through an affected individual's life with Huntington's disease or myotonic dystrophy. Broad ranges of repeat instability arise between individuals with expanded repeats, suggesting the
Laura Belvederesi et al.
Human mutation, 29(11), E296-E309 (2008-09-11)
Hereditary Non-Polyposis Colorectal Cancer (HNPCC) is associated with germline mutations in one of several MisMatch Repair (MMR) genes. An increasing proportion (20-25%) of the reported MSH2 variants consists of single amino-acid substitution with uncertain disease-causing significance. The present study was
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