MABE1807
Anti-NuMA1 Antibody, clone AD6-1
clone AD6-1, from mouse
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Nuclear mitotic apparatus protein 1, NuMA protein1, Nuclear matrix protein-22, NMP-22, SP-H antigen
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AD6-1, monoclonal
species reactivity
human, mouse
packaging
antibody small pack of 25 μg
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... NUMA1(4926)
Related Categories
General description
Nuclear mitotic apparatus protein 1 (UniProt: Q14980; also known as NuMA protein, Nuclear matrix protein-22, NMP-22, SP-H antigen) is encoded by the NUMA1 (also known as NMP22, NUMA) gene (Gene ID: 4926) in human. NMP-22 is a homodimeric nuclear protein that is highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role and occupies the majority if the nuclear volume. It is required for maintenance and establishment of the mitotic spindle poles during symmetric cell divisions, functioning as a tether linking bulk microtubules of the spindle to centrosomes. It is also considered to be essential or proper alignment of the mitotic spindle during asymmetric cell divisions. NMP-22 resides in the nuclear matrix during interphase and then dissociates from condensing chromosomes during early prophase and relocates to the spindle poles via dynein/dynamin association and it remains there until the anaphase onset. As mitosis progresses it re-associates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident. NMP-22 is phosphorylated in the C-terminal tail during mitosis, most likely by CDK1 and phosphorylation increases its solubility and promotes association with dynein and subsequent translocation to the spindle poles. O-glycosylation during cytokinesis at sites identical or close to phosphorylation sites interferes with the phosphorylation status of NMP-22.
Specificity
Clone AD6-1 detects NuMA1 in human and mouse cells. It targets an epitope within 181 amino acids from the C-terminal region.
Immunogen
A recombinant fragment corresponding to 181 amino acids from the C-terminal region of human Nuclear mitotic apparatus protein 1 (NuMA1).
Application
Anti-NuMA1, clone AD6-1 Antibody, Cat. No. MABE1807, is a highly specific mouse monoclonal antibody that targets NUMA1 and has been tested in Immunocytochemistry and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected NUMA1 in 10 µg of NIH/3T3 cell lysate.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected NUMA1 in HepG2 cells.
Immunocytochemistry Analysis: A representative lot detected NUMA1 in Immunocytochemistry applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).
Western Blotting Analysis: A representative lot detected NUMA1 in Western Blotting applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected NUMA1 in HepG2 cells.
Immunocytochemistry Analysis: A representative lot detected NUMA1 in Immunocytochemistry applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).
Western Blotting Analysis: A representative lot detected NUMA1 in Western Blotting applications (Carminati, M., et. al. (2016). Nat Struct Mol Biol. 23(2):155-63; Gallini, S., et. al. (2016). Curr Biol. 26(4):458-69).
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NUMA1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected NUMA1 in 10 µg of HeLa cell lysate.
Target description
~250 kDa observed; 238.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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