MABE144
Anti-Ago3 Antibody, clone 4B1-F6
clone 4B1-F6, from mouse
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Synonym(s):
eIF-2C 3, eIF2C 3, FLJ12765, MGC86946
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4B1-F6, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AGO3(192669)
General description
Eukaryotic translation initiation factor 2C 3, Ago3, belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain.
Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
Ago3 is indicated to play a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. Unlike Ago1 and Ago2, Ago3 and AG04 do not possess the ability to dissociate miRNA duplexes in a minimal in vitro system. Re-introduction of any Ago variant into embyronic (ES) cells deficient for expression of all four Ago variants will rescue miRNA silencing defects and reduce apoptosis.
Immunogen
Recombinant protein corresponding to human Ago3.
Application
Anti-Ago3 Antibody, clone 4B1-F6 is a Mouse Monoclonal Antibody for detection of Ago3 also known as eIF-2C 3, eIF2C 3, FLJ12765, MGC86946 & has been validated in ICC & IP.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HeLa cells. (Azuma-Mukai, A., et al. (2008). Proc Natl Acad Sci. USA. 105(23):7964-7969.)
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Ago3 in HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Ago3 in HeLa cells.
Target description
96 kDa calculated.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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