MABE143
Anti-Ago1 Antibody, clone 4G7-E12
clone 4G7-E12, from mouse
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Synonym(s):
hAgo1, Argonaute1, eIF-2C 1, eIF2C 1, Q99, GERP95, DKFZp686M13167
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4G7-E12, monoclonal
species reactivity
human
technique(s)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AGO1(26523)
General description
Ago1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
Immunogen
GST-tagged recombinant protein corresponding to human Ago1.
Application
Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.
Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.
Target description
~97 kDa observed.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Roberta Tarallo et al.
Genome biology, 18(1), 189-189 (2017-10-12)
The RNA-binding protein Argonaute 2 (AGO2) is a key effector of RNA-silencing pathways It exerts a pivotal role in microRNA maturation and activity and can modulate chromatin remodeling, transcriptional gene regulation and RNA splicing. Estrogen receptor beta (ERβ) is endowed
Catalina Méndez et al.
Retrovirology, 15(1), 67-67 (2018-10-06)
Current antiretroviral therapy is effective in controlling HIV-1 infection. However, cessation of therapy is associated with rapid return of viremia from the viral reservoir. Eradicating the HIV-1 reservoir has proven difficult with the limited success of latency reactivation strategies and
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