MABE1154
Anti-Fibrillarin Antibody, clone 38F3
clone 38F3, from mouse
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Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
38F3, monoclonal
species reactivity
human, rat, yeast, mouse, Drosophila
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... FBL(2091)
General description
rRNA 2′-O-methyltransferase fibrillarin (UniProt: P15646; also known as Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1) is encoded by the NOP1 (also known as LOT3, YDL014W, D2870) gene (Gene ID: 851548) in yeast. Fibrillarin is a nucleolar protein that is highly conserved throughout evolution. Human fibrillarin is 67% identical to its yeast homolog Nop1p. Fibrillarin is a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs) that direct 2 -O-methylation of rRNA and is also involved in other aspects of rRNA processing. Unlike snRNAs, which have a cytoplasmic phase of maturation, snoRNAs are restricted to the nucleus. Fibrillarin is essential for yeast viability and fibrillarin knockout is shown to be lethal in S. cerevisiae; however, expression of human or Xenopus fibrillarin overcome this lethality. The first 80 amino acids from the N terminal region of fibrillarin contain multiple RGG, or arginine-glycine-glycine (GAR). The remaining ~240 amino acids consist of the so called fibrillarin domain. NOP1 depleted strains of yeast are greatly impaired in the production of cytoplasmic ribosomes, and they have a reduced level of rRNA. Patients with scleroderma are reported to have strong circulating autoantibodies to fibrillarin. (Ref.: Tollervey, D et al. (1991). EMBO J. 10(3):573-83; Newton, K et al. (2003). Mol. Cell. Biol. 23(23): 8519-8527).
Specificity
Clone 38F3 specifically detects Fibrillarin/Nop1p in multiple mammalian species and in yeast.
Immunogen
Yeast nuclear extract
Application
Anti-Fibrillarin, clone 38F3, Cat. No. MABE1154, is a highly specific mouse monoclonal antibody that targets Fibrillarin/Nop1p and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected Fibrillarin in human SH-SY5Y cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected Fibrillarin in Immunocytochemistry applications (Newton, K., et. al. (2003). Mol. Cell. Biol. 23(23):8519-27).
Immunocytochemistry Analysis: A representative lot detected Fibrillarin in Immunocytochemistry applications (Newton, K., et. al. (2003). Mol. Cell. Biol. 23(23):8519-27).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Quality
Evaluated by Western Blotting in HeLa cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibrillarin in 10 µg of HeLa cell lysates.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibrillarin in 10 µg of HeLa cell lysates.
Target description
~34 kDa observed; 34.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Affinity Purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Regulatory Information
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