MABD411
Anti-BMP4 Antibody, clone PA354-16.1.1
clone PA354-16.1.1, from mouse
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Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PA354-16.1.1, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BMP4(652)
Related Categories
General description
Bone morphogenetic protein 4 (BMP4), also known as Bone morphogenetic protein 4 (BMP-4), Bone morphogenetic protein 2B (BMP-2B), and encoded by the gene name BMP4/ BMP2B/ DVR4, is morphogenetic protein that induces cartilage and bone formation. Similar to other bone morphogenetic proteins, BMP4 has been shown to play an important role in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
Immunogen
MBP-tagged recombinant protein corresponding to human BMP4.
Application
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BMP4 in human pancreas tissue.
Flow Cytometry Analysis: 1:200 dilution of this antibody from a representative lot detected BMP4 in Caco-2 cells (Data courtesy of Juan Ignacio Imbaud, Protein Alternatives).
Flow Cytometry Analysis: 1:200 dilution of this antibody from a representative lot detected BMP4 in Caco-2 cells (Data courtesy of Juan Ignacio Imbaud, Protein Alternatives).
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
This Anti-BMP4 antibody is validated for use in WB, FC, IH for the detection of BMP4.
Quality
Evaluated by Western Blotting in human recombinant protein BMP4.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.01 µg human recombinant protein BMP4.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected 0.01 µg human recombinant protein BMP4.
Target description
47 kDa calculated. The calculated molecular weight is 47 kDa, however BMP4 has been shown as a ~80 and ~90 kDa band in western blots with the use of a MBP tag.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
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mAbs, 8(4), 678-688 (2016-03-12)
Due to improved understanding of the role of bone morphogenetic protein 4 (BMP4) in an increasing number of diseases, the development of selective inhibitors of BMP4 is an attractive therapeutic option. The currently available BMP4 inhibitors are not suitable as
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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