Anti-Sortilin Antibody, clone 4E3.1

Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes. Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage. Sortilin is initially produced with a signal peptide (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region (a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.

Expected to react with both spliced isoforms reported by UniProt (Q99523).

Epitope: C-terminal region.

GST-tagged recombinant human Sortilin C-terminal fragment.

Anti-Sortilin Antibody, clone 4E3.1 is an antibody against Sortilin for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: An 1:250 dilution of this antibody from a representative lot detected Sortilin in human cerebral cortex, colon, and kidney tissue sections.

Research Category
Stem Cell Research

Research Sub Category
Vesicular Trafficking

Evaluated by Western Blotting in E13-14 embryonic mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sortilin in 10 µg of E13-14 embryonic mouse brain tissue lysate.

~92 kDa observed. Target band size appears larger than the calculated molecular weights of 83.86 kDa (isoform 1) and 69.17 kDa (isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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