MABD20
Anti-PAX7 Antibody, clone 2F12H4
clone 2F12H4, from rat
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paired box 7, PAX7 transcriptional factor, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2F12H4, monoclonal
species reactivity
mouse, human (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039)
technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Pax7(18509)
General description
Paired box protein Pax-7 (PAX7) belongs to the Pax family of transcription factors, a highly conserved group of proteins with crucial roles in tissue homeostasis and organogenesis. PAX7 is involved in the specification, survival, maintenance, and self-renewal of skeletal muscle progenitors and myogenic progenitors. It is primarily expressed in newly activated and quiescent satellite cells. Studies have shown that on activation, quiescent satellite cells co-express MyoD with PAX7 prior to proliferation, after which one or the other transcription factor is downregulated. Downregulation of MyoD instead of PAX7 leads to withdrawal from the cell cycle and myogenic differentiation, causing the cell to return to seemingly quiescent state. Chromosomal aberrations relating to PAX7 have been linked to rhabdomysarcoma 2 (RMS2).
Immunogen
KLH-conjugated linear peptide corresponding to mouse PAX7.
Application
Anti-PAX7 Antibody, clone 2F12H4 is a Rat monoclonal antibody for detection of PAX7 also known as paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7 & has been validated in WB, ICC.
Immunocytochemistry: A previous lot of this antibody was shown to detect Pax7 localized in euchromatin of human Ewing’s
sarcoma cells. The antibodies are detected with Alexa Fluor™ 488-conjugated anti-rat IgG. Each sample is counterstained with hoechst (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039).
sarcoma cells. The antibodies are detected with Alexa Fluor™ 488-conjugated anti-rat IgG. Each sample is counterstained with hoechst (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039).
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in mouse fetal brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PAX7 in 10 µg of mouse fetal brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected PAX7 in 10 µg of mouse fetal brain tissue lysate.
Target description
~ 55 kDa observed.
The calculated MW of PAX7 is 55 kDa; however, expression of PAX7 can have multiple isoforms. (Robert B., et al. (2008). Physiol. Genomics 33:41-49.)
The calculated MW of PAX7 is 55 kDa; however, expression of PAX7 can have multiple isoforms. (Robert B., et al. (2008). Physiol. Genomics 33:41-49.)
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse fetal brain tissue lysate
Mouse fetal brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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