MABC946
Anti-PKCζ Antibody, clone 14B12.3
clone 4B12.3, from mouse
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Protein kinase C zeta type, nPKC-zeta, PKC-zeta, PKCζ
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4B12.3, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRKCZ(5590)
mouse ... Prkcz(18762)
rat ... Prkcz(25522)
General description
Protein kinase C zeta type (EC 2.7.11.13; UniProt Q05513; also known as nPKC-zeta, PKC-zeta) is encoded by the PRKCZ (also known as PKC2) gene (Gene ID 5590) in human. Protein kinase Cs (PKCs) are threonine/serine kinases that play important roles in cellular signal transductions. PKC isozymes are classified into three major classes based on their domain compositions and activator requirements. The conventional PKCs (cPKCs) include the alpha, beta I, beta II, and gamma isoforms. The novel PKCs (nPKCs) include the delta, epsilon, eta, and theta isoforms. Atypical PKCs (aPKCs) include the lambda (mouse ortholog to human PKC-lambda is called PKC-iota) and zeta isoforms. Both cPKCs and nPKCs bind diacylglycerol (DAG) via their C1 domain, while only cPKCs, but not nPKCs bind Ca+2 via their C2 domain. The aPKCs do not bind either DAG or calcium for activity. All three classes of PKCs contain a pseudosubstrate sequence in their N-terminal region that keeps the substrate-binding cavity occupied via intramolecular interaction when the enzymes are in their inactive conformation. Conformation change upon enzyme activation frees the substrate-binding site.
Specificity
Expected to react with all three spliced isoforms of human PKC-zeta.
Immunogen
Epitope: C-terminal region
GST-tagged recombinant human PKCζ C-terminal fragment.
Application
Anti-PKCζ Antibody, clone 14B12.3 is an antibody against PKCζ for use in Western Blotting, Immunohistochemistry (Paraffin).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected PRKCZ in 10 µg of PC12 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PRKCZ in human cerebral cortex, human cerebellum and mouse brain tissues.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PRKCZ in human cerebral cortex, human cerebellum and mouse brain tissues.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PKCζ in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PKCζ in 10 µg of mouse brain tissue lysate.
Target description
~70 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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